Development and Validation of RP-HPLC Method for the Simultaneous Determination of Etoposide and Cisplatin and its Application in Quality Control of Injectable Dosage Forms

Etoposide and cisplatin are antineoplastic drugs used in injectable dosage forms in many kinds of cancer separately or in combination therapy. The objective of the work was to develop a simple and rapid RP-HPLC method for direct simultaneous determination of etoposide and cisplatin in injectable dosage forms, aiming its application in quality control analysis. The HPLC method was developed using C18 ODS Hypersil column of 250 × 4.6 mm id with 5 µm particle size, mobile phase of water : methanol : acetonitrile (40:35:25, v/v/v, pH 3.5) at a flow rate of 0.75 mL min-1 and eluate dete..read more

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JAMEEL RAHMAN ,IRSHAD AHMAD ,MUHAMMAD SALEEM ,MUHAMM..

Development and Validation of RP-HPLC Method for Determination of Famotidine and its Application in Quality Control of Different Pharmaceutical Dosage Forms

A precise and fast novel high-performance liquid chromatography method was developed and validated for the quantitative determination of Famotidine (FMT) in commercially available pharmaceutical dosage forms. An Agilent 1200 Series High Performance Liquid Chromatography (HPLC) system having the column C18 (5µm particle size, 150×4.6 mm) was used in this study and detection (diode array detector) was made at 280 nm. The mobile phase was acetonitrile, distilled water, triethylamine and phosphoric acid (49.9:49.9:0.1:0.1, v/v), isocratic elution under ambient temperature at flow rate of 1.5 mL mi..read more

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SYED SAEED UL HASSAN, IRSHAD AHMAD, MUHAMMAD AYUB, SAIQ..