The presented work is focused on the investigation of ferrocene and its derivatives (ferrocene benzoic acid and ferrocene dicarboxylic acid) via electrochemical, and spectrophotometric for biological activity. DNA binding capacity was studied by cyclic voltammetry and UV-Vis spectroscopy with evaluation of kinetic and thermodynamic parameters. Heterogeneous rate constant and diffusion coefficients were estimated for pure compounds and the compound bound with ds.DNA (double-stranded DNA). The voltammetric investigation suggested that the interaction between the ferrocene derivatives and ds.DNA was present. Oxidation potential (Ep) values showed that the interactions of ferrocene dicarboxlic acid (FC-2) with ds.DNA was stronger than that of ferrocene benzoic acid (FC-1). Diffusion coefficient and heterogeneous rate constant values also corresponded to the structural dependent interactions of these compounds with ds.DNA.

Asifa Nigar, Naveed Kausar janjua, Asghari Gul, Abid Ali, Zareen Akhter, Sadia Mehmood