Nicotinic acid and Nicotinamide were separated, identified and determined in cigarette tobacco and smoke by reverse phase HPLC with UV detection using water methanol (1:1) as mobile phase. Optimum solvent, volume of solvent, temperature and time were investigated for the extraction of nicotinic acid and nicotinamide from tobacco. All available brands of cigarettes tobacco and cigarette smoke were analyzed at optimum conditions and the results are presented and discussed.
M RASUL JAN ,JASMIN SHAH ,FAHMIDA GUL ,ABIDULLAH SHAH ,
You can use below filter to find your desired issue