The present study involved functional investigation of the signalling pathway of adenylyl cyclase inhibition by the muscarinic receptor. Methacholine pre-treatment of tracheal smooth muscle resulted in inhibition of isoprenaline- and guanine nucleotide-stimulated adenylyl cyclase. The effect could be reversed by pre-incubation of the tissue with the antibody that identifies the C-terminal decapeptide of inhibitory G proteins 1 and 2 (G(i)1 and G(i)2) indiscriminately. Methacholine also abated the pertussis toxin-catalysed ADP-ribosylation of G(i alpha). These results suggest the existence of M-2 and/or M-4 subtype of the muscarinic receptor in the tracheal smooth muscle of guinea-pig.
DARAKHSHANDA SHEHNAZ ,IAN W RODGER ,WILLIAM C BOWMAN ,
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