A new method for separation and speciation of organophosphorus pesticides have been investigated using reverse phase HPLC. Pesticides are hydrolysed by the addition of sodium ethoxide, the organic layer is separated from the aqueous phase which contain the acidic hydrolysed portion of the respective pesticides. Different pesticides give different acidic products on hydrolysis leading to variation in the retention time. The hydrolysed products are injected and separated on reverse phase HPLC. Identification and quantification is done by running the respective standards. 


M RASUL JAN ,KAHKESHAN SAEED ,JASMIN SHAH ,KAHKESHAN SAEED ,M RASUL JAN ,