Production of Glucoamylase by Fusarium moniliforme under Solid-State Fermentation

Production of glucoamylase employing Fusarium monilforme under solid-state fermentation was optimized. Different substrates, like rice bran, green gram bran, black gram bran, wheat bran and maize bran, were investigated to select the best substrate. Accordingly, wheat bran showed the highest glucoamylase activity; the maximum glucoamylase activity under optimal conditions was 96.59 ± 3.56 UI g of wheat bran. The optimum conditions were: yeast extract as additive I % (w/w), incubation period of 96 h, incubation temperature 30 ± 2°C, inoculum level 4 ml, moisture content of the wheat bran 60 %, and amount of wheat bran was I5 g at pH 5.0.  

M ASGHER ,G MUSTAFA ,H N BHATTI ,