Peptide Synthesis Catalyzed by Immobilized Trypsin in Organic Solvents

Trypsin was immobilized to poly vinyl alcohol (PVA) by adsorption from its aqueous solutions. The catalytic activity of trypsin increased markedly by irnmobiliZl!-tion for peptide synthesis from N-a-benzoyl L-arginine in hydrophilic organic solvents such as tetrahydrofuran or ethanol. The yield of Bz-Arg-Leu-NH2 is strongly dependent on the PV Ntrypsin ratio and water content in the reaction medium. The optimum pH and temperature were determined for immobilized trypsin as 7.0 and 40°C, respectively

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