In the present study, the crude polysaccharides from pomegranate peels (CPP) were prepared by compound enzyme-extraction technology, and the response surface methodology was used to optimize the extraction parameters. Box–Behnken design (BBD) was applied to estimate the effects of extraction temperature, extraction pH, and dosage of compound enzyme on the yield of CPP. A mathematical model with high fitness was obtained. Extraction temperature, extraction pH, and dosage of compound enzyme exhibited independent and interactive effects on CPP yield. CPP were purified by macroporous resin HP-20 and Sephadex G-100 column chromatography to afford purified fraction of CPP-2. The relative molecular weight of CPP-2 was 93.5 kDa. In CPP-2, and FT-IR spectra showed that the main components among CPP-2 may be pectic polysaccharides.The effect of CPP-2 on angiogenesis was measured in vivo by using the chick embryo chorioallantoic membrane (CAM) assay. The results demonstrated that CPP-2 suppressed angiogenesis in chicken embryos.
Chunlin Ke and Zuomei Li
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