Normal 0 false false false /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} Diazepam reacts with ninhydrin at pH range 12-12.5 to give a violet color having maximum absorbance at 530 nm wavelength. The reaction is specific for diazepam and provides a basis for its spectrophotometric determination. The color reaction obeys Beer’s law from 0.1 to 2 mg/10 mL of diazepam. Coefficient of variation and confidence limit determination are 0.06 and 0.0041%, respectively. The relative standard deviation is 1.5%. The quantitative assessments of tolerable amount of other drugs have also been studied.
NAEEM ASGHAR KHAN ,NARJIS NAZ ,NADEEM ASGHAR KHAN ,NAVEED ASLAM DOGER ,WASEEM ASGHAR KHAN ,
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