Ciprofloxacin HCl, 1-Cyclopropyl-6-fluoro-4-oxo-7-(piperazin1-yl)-1,4-dihydroquinoline-3-carboxylic acid, is a synthetic broad antibacterial compound belonging to the group of fluoroquinolones which is antimicrobial drug act through the inhibition of DNA-gyrase, an enzyme that is critical to bacterial chromosome replication. It is used in a wide range of infections of the urinary, respiratory and gastrointestinal tracts, as well as in skin structure and ocular infections. Levofloxacin Hemihydrate is a fluoroquinolone, has a broad-spectrum antibiotic activity against gram-positive and gram-negative bacteria and used in respiratory and urinary tract infections. Various Pakistani branded antibiotic drug samples containing Ciprofloxacin HCl and Levofloxacin Hemihydrate were collected from local market and their working standards from the material suppliers of Karachi city. Samples were analyzed by using UV-Vis spectrophotometer. Then the method was validated by linearity, accuracy, precision (reproducibility, intermediate precision) and range which suggested that method is appropriate and proper to determine the potency of these various brands of Ciprofloxacin HCl and Levofloxacin Hemihydrate. The analytical data suggest that the drug samples analyzed were within the limit of the Pharmacopeia requirement and criteria.

Mir Munsif Ali Talpur, Tajnees Pirzada and Muhammad Afzal Arain