Capillary gas chromatography (GC) was examined from the determination of urinary putrescine(Pu) and cadaverine (CA) based on off line precolumn derivatization with trifluoroacetylacetone (FAA), GC was carried out on BP5 (25m x 0.22 mm id) column with a layer thickness of 0.25 mum at 230 degreesC with a heating rate of 2 degreesC/min upto 250 degreesC, a nitrogen flow rate of 3.5 ml/min, a split ratio of 1:15 and detection by FID. The method was used for determinastion of Pu and CA in urine of cancer patients before and after radiotherapy and healthy volunteers. The amount found was in the range of 0.04-3.89 mug/ml with a coefficient of variation (C.V) of 1.25-5.56%.
M Y KHUHAWAR ,A A MEMON ,M I BHANGER ,
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