Enzymes bound on solid supports have number of advantages over their soluble counterparts e.g. high stability, no purification, easy handling, economicity and high yield of product. Immobilised enzymes have gained increased signifinance in several areas of analytical chemistry. The use of immobilised enzymes is not only limited for the determination of their substrates but is also gaining attention for the determination of many enzyme inhibitors. This paper gives brief description about the introduction of immobilised enzymes, methods of immobilisation and their analytical applications particularly in connection with flow injection analysis (F.I.A.).