An HPLC method with high precision, accuracy and selectivity was developed and validated for the assessment of cephradine in human plasma samples. The extraction procedure was simple and accurate with single step followed by direct injection of sample into HPLC system. The extracted cephradine in spiked human plasma was separated and quantitated using reversed phase C18 column and UV detection wavelength of 254 nm. The optimized mobile phase of new composition of 0.05 M potassium dihydrogen phosphate (pH 3.4)-acetonitrile (88:12) was pumped at an optimum flow rate of 1 mL.min-1. The method resulted linearity in the concentration range 0.15-20 µg.mL-1. The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.150 µg.mL-1, respectively. The accuracy of method was 98.68 %. This method can be applied for bioequivalence studies and therapeutic drug monitoring as well as for the routine analysis of cephradine.
SATTAR BAKSH ,MAHMOOD AHMAD ,NAVEED AKHTER ,MUHAMMAD USMAN ,WAHEED ASGHAR ,ASADULLAH MADNI ,NAYAB KHALID ,
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