A fast flow system for the quantitative determination of lipase is described Crude lipase preparation from porcine pancreas is used. The substrate used is p-nitrophenyl laurate emulsion in polyvinylalcohol, p-nitrophenol is quantitated spectrophotometrically at 405 nm. The detection limit is 0.1 U ml(-1) and the sample throughput is 25 hr(-1). The relative standard deviation for 10 injections of 92.5 U ml(-1) was 1.5%.
M MASOOM ,M YAQOOB ,M ANWAR ,
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