Inquiries of DNA Interaction of Cobalt (II) Phthalocyanine Compound Bearing 4-tritylphenoxy Groups

[Tetrakis-(4-tritylphenoxy) phthalocyaninato] Cobalt (II) (PcCo) was previously formed via the reaction of 4-(4-tritylphenoxy)phthalonitrile. The structure of the resulting PcCo was characterized using absorption spectra, infrared and NMR spectroscopies. The deoxyribonucleic acid (DNA) connecting feature for PcCo was inquired in the different concentration of Calf Thymus-deoxyribonucleic acid (CT-DNA) using UV/Vis, fluorescence spectroscopy, gel agarose electrophoresis and thermal denaturation methods. UV/Vis spectrometer and fluorescence spectroscopy verified PcCo bounds to the DNA and the binding constant (Kb) of the PcCo was also calculated. The binding constant Kb is a very important parameter for getting information on the binding mechanism. The Kb of the compound was also calculated as 1.44 x 106 M-1. The value of Kb demonstrated that the complex binds to DNA through an intercalative binding mechanism. Additionally, thermal denaturation and the electrophoresis studies were implemented to analyze the interacting of PcCo via CT-DNA. In the absence of the complex, the melting experiments were conducted for CT-DNA and Tm was found 69.6 oC for the DNA and The Tm of PcCo complex was found 76.3 oC. Melting point temperature and electrophoresis experiments demonstrated that PcCo binds to CT-DNA through intercalation binding mechanisms. The acquired findings confirmed that PcCo links to the DNA via the intercalating binding manner. Therefore, PcCo may have potential use in cancer treatment. Because of this, further research is needed before this complex can be used in cancer treatment

Ali Arslantaş and Mehmet Salih Ağırtaş