Vitamin K3 (menadione sodium hydrogen sulphite) was indirectly determined by the reduction of the iron (III) to iron(II) and an intense blue color formation by the reaction of iron(II) and 2,4,642'-pyridyl)-e4riazine (TFTZ). The color development was monitoredspectrophotometerically at 595 ma with linear calibration range with 2-10 µg/mi of vitamin K3 The method was used for the determination of vitamin K3 in a pharmachemical preparation A single lined flow injection manifold was also examined. A large excess of iron(III) containing different concentration of vitamin K was run, on the line and a constant amount of TPTZ was injected through the injector. The peak height was proportional to concentration between 2-10 µg/ml vitamin K3.
F M A RIND ,S A MEMON ,MUHAMMAD Y KHUHAWAR ,
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