Indirect Determination of Vitamin K3 Using 2,4,6-tri.s(2'-Pyridyl)-S-Triazine by Spectrophotometeric and Flow Injection Techniques

Vitamin  K3 (menadione  sodium hydrogen   sulphite)   was   indirectly determined by the reduction  of the    iron (III)  to iron(II)    and  an intense  blue  color formation by  the  reaction  of iron(II)     and   2,4,642'-pyridyl)-e4riazine   (TFTZ).   The  color   development     was    monitoredspectrophotometerically  at 595  ma with  linear calibration range  with  2-10 µg/mi  of vitamin K3 The method  was  used  for the  determination of vitamin K3 in a pharmachemical preparation   A single    lined  flow injection   manifold  was  also examined. A large   excess  of iron(III) containing  different concentration    of vitamin K    was  run, on the line and  a constant amount of TPTZ was  injected through    the injector. The peak height  was proportional  to concentration between  2-10  µg/ml vitamin K3.

F M A RIND ,S A MEMON ,MUHAMMAD Y KHUHAWAR ,