T1DM is the most acquainted childhood immune-caused onset and may lead to early mortalities and morbidities. It can emanate in any age group but is highest reported in lesser than 18 years of age. In Pakistan only, T1DM institutes ~ 2% of the all diabetic population. This work was formulated to assess the concentration of manganese (Mn), zinc (Zn) and chromium (Cr) in blood, scalp hair and serum samples of diabetic mellitus type 1 children age ranged (1–5), (6-10) and (11-14) years of both genders (male and female), compared with similar sex and age referent subjects. For sample pretreatment, microwave-aided acid digestion procedure (MAD) was employed for elemental analysis in the biological samples of T1DM children. The employed method was validated by using certified reference samples of blood, scalp hair, and serum. Digests biological specimen were analyzed by AAS. The Zn was analyzed by FAAS, while the assessment of Mn and Cr were measured by ETAAS. The Mn, Zn and Cr, presents significantly low levels in the samples of scalp hair, blood and serum of female and male of age group of (1-5) (6-10) and (11-14) of diabetic mellitus type 1 children, compared with referent subjects. These data present an important hypothesis to doctors and other clinical experts to investigate insufficiency of these essential micronutrients (Cr, Mn, & Zn) in biological specimen of blood, scalp hairs and serum of T1DM children.

Anjum Lashari, Hasan Imran Afridi, Tasneem Gul Kazi, Farah Naz Talpur and Jameel Ahmed Baig