By studying cross-linking of poly-N-vinylpyrrolidone [Mn=1×104 and 4×104] with N,N`-methylene-bis-acrylamide there were obtained water-swelling gel-forming polymers. Also was studied immobilization process of trypsin in polymer gels and Co(II), Cu(II), Ni(II) metal gel-complexes in polymeric gels. It was found that the amount and activity of immobilized trypsin gel carriers based on poly-N-vinylpyrrolidone less compared to the amount and the activity of immobilized trypsin gel carrier cross-linking copolymer of N-vinylpyrrolidone with arabinogalactan from cherry gum. The immobilization mechanism of trypsin into polymer gels investigated by FTIR and 1H NMR spectroscopy.


Shamo Zokhrab Tapdiqov, Nizami Allahverdi Zeynalov, Dilgam Babir Taghiyev, Saadat Faik Humbatova, Samira Mirali Mammedova, Elnara Fakhraddin Nasiyyati and Dilshad Talib Babayeva