The electrochemical fingerprints of Rhizome atractylodis macrocephalae were studied by B–Z (Belousov-Zhabotinski) oscillation system of H+-Mn2+-CH3COCH3-BrO3－. The influences of temperature, stirring rate, hydrogen ion concentration, Rhizome atractylodis macrocephalae dosage, etc, to electrochemical fingerprints of Rhizoma atractylodis macrocephalae were investigated. The optimum conditions were established with total volume of 100 mL, temperature of 37℃, stirring rate of 400 rpm, hydrogen ion concentration of 1.980 mol•L-1, Rhizoma atractylodis macrocephalae dosage of 1.000 g, KBrO3 concentration of 0.05000 mol•L-1, MnSO4 concentration of 0.005600 mol•L-1 and CH3COCH3 of 1.00 mL by comparing difference of the electrochemical fingerprints of Rhizoma atractylodis macrocephalae from two choosen regions. The results indicated that the electrochemical fingerprints of Rhizoma atractylodis macrocephalae from different origins showed significantly different characteristics, so the electrochemical fingerprint is a simple method to identify the origins and variety of Rhizoma atractylodis macrocephalae.
Gen-Lian Bao, Jian Zhong Jin, Hang-Shi Xu and Zhong-Yang Wang
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