Removal of hydrogen sulfide (H2S) has been attempted from various distillation fractions with bacterium Pseudomonas putida. Biodesulfurization technique has been found to be more effective than conventional desulphurization methods. Tremendous amount of hydrogen sulfide (H2S) has been removed in all the distillation fractions under study i,e. 64.94 %, 65.83 % and 66.66 % has been reduced in case of  light (bp 200 0C), medium (bp 300 0C),  and heavy (bp 360 0C) distillate fractions, respectively. Because of the volatile nature, hydrogen sulfide (H2S) was found to be concentrated mostly in light distillate (0.0170 %) next in medium (0.0120 %) and then in heavy (0.0090 %) distillate fractions


MUHAMMAD SHAHKIRULLAH ,MUHAMMAD ISHAQ ,MUHAMMAD NAEEM ,IMTIAZ AHMED ,MUHAMMAD ARSALAN KHAN ,