An improved ion chromatographic OC) method has been developed for the determination of magnesium and calcium in high purity uranium The extraction of alkaline earth metal ions present in samples were optimized Icing tributyl phosphate (TBP) as an extractant Excellent recoveries of 99% were achieved for investigated alkaline earth metals at trace level. The relative standard deviations (RSD'S) for Mg2+ and Ca2+ ions for the ion chromatographic method were 0.5 and 0.8 ppm respectively. The analytes were also analyzed with atomic absorption spectroscopy (AAS) as a counter technique.