A flow injection spectrophotometric method is developed for the quantitative determination of phosphorylcholine utilizing alkaline phosphatase reactor (immobilized on controlled-pore glass). The measurement is based on the rate of formation of ''Molybdenum blue'', a heteropoly molybdenum (V) species at 660 nm. The detection limit is 10 mu M at a sampling rate of upto 45 hr(-1), with a relative standard deviation of lower than 1% (n=10).
M YAQOOB ,M MASOOM YASINZAI ,G HUSSAIN ,
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