The ESR Method to Detect the Irradiated Fish and Beef Bones

Electron spin resonance (ESR) spectroscopy was employed for identification and quantification of the more stable free radicals generated by irradiation in bones of fish and beef. The samples were irradiated 1-5 kGy with gamma rays using Co-60 source. ESR spectroscopy was employed to detect irradiation in samples of fish and beef bones.  The results revealed that the irradiated samples showed strong asymmetric signals with g1 2.002 ± 0.001 and g2 1.998 ± 0.001 respectively, which was a clear indication of irradiation. The control samples were not irradiated and showed typical low intensity symmetric signals at g-value of 2.005 ± 0.001 revealing the absence of radiation treatment. It was also observed that intensities of signals due to hydroxyapatite radicals for 5 kGy were normally higher than 1 kGy radiation dose, showing the dose dependency.

SHAHEEN ATTA ,SAEED A NAGRA ,TAUFIQ AHMED ,AURANG ZEB ,