The UV and visible absorption characteristics of thiamine hydrochloride, riboflavin and ascorbic acid have been studied in pH range 1.0-11.0 in relation to the photochemical interaction of thiamine Hydrochloride/ascorbic acid with riboflavin due to overlapping of their adsorption bands in UV region. The spectral variation in thiamine hydrochloride and ascorbic and solutions on `photolysis in the presence and absence of riboflavin have been monitored and the effects of pH of the nature of these variations has been discussed. A comparison of the magnitude of spectral variations in the presence and absence of riboflavin indicates that the photolysis of thiamine hydrochloride is inhibited by riboflavin whereas that of ascorbic acid is promoted by riboflavin. The non-ionized forms of thiamine hydrochloride and ascorbic acid appear to be less susceptible to photolysis than the ionized forms. Thiamine hydrochloride and ascorbic acid are more stable to photo degradation in the acid range in the presence and absence of riboflavin.
R H SHAIKH ,F H M VAID ,I A ANSARI ,I AHMAD ,
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