: Physico chemical parameters of soil samples collected from agriculture lands of Taluka Sobhodaro District Khairpur, Sindh, Pakistan were examined to check the quality of soil. Moisture contents percentage, pH and EC values indicated that texture of soils is mostly silt- loam type having water holding ability hence can be considered as good fertile land, beneficial for crops. Humic acids (HAs) were extracted from soil samples by International Humic Substance Society(IHSS) method. HAs form biogeochemical cycle due to presence of electron clouds resultant macro and micro nutrients, transferred to the plants. HAs obtained were characterized by UV-Visible spectroscopy. The optical parameter E4/E6 values illustrated that HAs possess high molecular condensation and proved to be hydrophilic and aromatic in nature. To analyze the binding ability with metal ions, isolated HAs were complexed with potassium, zinc and ferric ions under optimized conditions. The concentrations of selected metal ions were detected in complexed HAs samples by Atomic Absorption Spectrophotometer (AAS). The data obtained indicated that the maximum concentration of complexation of metal ions was found for K1+at pH 8, Zn2+7 and Fe3+6. The data was correlated and compared with literature.
Tajnees Pirzada, Abdul Ghaffar Solangi, Mir Munsif Ali Talpur, WeengharAli Chandio and Khadija Memon
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