Phospholipase-A has been purified to homogeneity by combination of reverse-phase HPLC with gel permeation or ion-exchange HPLC. The amino acid composition and N-terminal sequence analysis has also been determined. A difference at position 10 has been observed from other species of Naja naja naja. The preparation is being employed for further structural characterization. A short peptide (MW 3kDa) has also been purified from the Pl-A containing fraction. The amino acid composition, N-terminal sequence, molecular mass by SDS-PAGE and mobility on PAGE has been determined. The peptide seems to represent a new type of small peptide in Cobra venom.
Z H ZAIDI ,O U BEG ,J SHAFQAT ,
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