Enantiomeric Separation by Capillary Electro chromatography with a Novel β-cyclodextrin-hyperbranched Carbosilane–silica Polymer Coating

A new type of β-cyclodextrin-modified hyperbranched carbosilane (β-CD@HBC) stationary phase was synthesized by combining hyperbranched carbosilane with the -OH group of β-cyclodextrin, and then the β-CD@HBC was used as astationary phase to fix it on the inner wall of capillary column, application in chiral drug separation. The chromatographic behaviour of the stationary phase was studied. The preliminary test results showed that the β-CD@HBC coated OT column has the ability to separate a variety of enantiomers, such as clenbuterol, ibuprofen, enrofloxacin, hlorpheniramine, isoproterenol hydrochloride, epinephrine, Phenylalanine, and Ketoprofen. The β-CD@HBC coated OT column has good repeatability and stability. The relative standard deviations (RSD) between runs, between days, and between columns were less than 1.5%, 1.86%, and 4.83%, respectively. This paper shows that hyperbranched polymers have great potential and advantages in the application of capillary electrochromatography for chiral separation.

Xiangqing Yang and Yali Hang