A new spectrophotometric flow injection analysis method has been successfully developed to measure chromium (III) ion levels. This approach relies on the oxidation of the chromium (III) ion by hydrogen peroxide in a basic environment, leading to the formation of the chromate ion. This ion subsequently reacts with 1,5-diphenylcarbazide in an acidic solution, resulting in a striking blue-violet complex. Under optimal conditions, the method demonstrates a linear response within the range of 0.05 to 15 mmol.L-1, achieving a high correlation coefficient (r) of 0.9912. The detection limit is impressively low at 51.996ng/100µL, and the precision, quantified by relative standard deviation (RSD%) across six replicate measurements, remains below 1% for Cr(III). This analytical technique has been effectively utilized to quantify chromium (III) ion content in three different pharmaceutical preparations: Vitaking kft-200µg from Hungary, GTF-200µg from the USA, and AdvaCare Pharma-200µg from the USA, all produced by different manufacturers using a homemade NAG-SSP analyzer. In comparing this novel method with a traditional spectrophotometric approach, statistical analysis revealed no significant differences between the two at a 95% confidence level, as determined by the paired t-test and one-way ANOVA. These results indicate that the new method can be confidently adopted as a reliable routine alternative for analyzing Cr(III) in various pharmaceutical products.
Marwah A. Kadhim Al-banaa, Nawras Majid Hassouni, Turkey, Nagham Shakir and Hussein Fares Abd-Alrazack
You can use below filter to find your desired issue