A simple HPLC procedure for the determination of fenoxaprop-p-ethyl herbicide in environmental samples is described. The chromatographic analysis was carried out by HPLC, on a C18 packed capillary column (4x4 mm,4.6 X 150 mm, 5mm particle size) with 20 µl injection volume and UV detector at 280 nm. HPLC-grade acetonitrile and methanol were used as mobile phase with flow rate of 1mL min-1 . Samples were spiked with amount between 5 - 20µg g-1 of herbicide and were isolated from samples by applying microwave assisted extraction (MASE) at ambient temperature. Percent recoveries were improved by optimizing solvent types, solvent volume, extraction temperature and time. Calibration curve range determined by HPLC was 0.5-16µg mL-1 .The interaction of different variables for maximum % recovery response was checked by applying factorial design and was found to be in range of 91.22±0.01—99.32±0.01 with good precision (< 5% ). Application of this procedure to the analysis of herbicide in ester and acid form showed the effectiveness of the proposed approach.
FARHAT-UN-NISA SHEHZAD, JASMIN SHAH, MUHAMMAD RASUL JAN AND MIAN MUHAMMAD
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