Radio-labeled antibiotics are being employed for the particular diagnosis of infections utilizing their specific binding properties to the bacterial components. Stable freeze dried kits of ciprofloxacin is developed through simple stirring at room temperature and labeled with 99mTc after incubation at 35°C.Kits produced through this method showed high stability both at room temperature and in serum at 37ºC. The radiochemical purity of the complex was 99.6% when 1.0 mg ciprofloxacin in the presence of 100.0 μg SnCl2, 2.0 mg gentistic acid, 3.0 mg penicillamine and 20.0 mg d-mannitol was labeled by 10-20 mCi sodium pertechnetate. Serum protein binding, lipophilicity, in-vitro binding with Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli as well as in-vivo biodistribution in infected rabbits were also investigated. Biodistribution results exhibited that radio ligand had good affinity in the infected site in rabbit. The uptake for Pseudomonas aeruginosa, Salmonella typhi and Escherichia coli induced infections as target to non-target ratio (T/NT) 5.425 ± 0.17, 5.397±0.15 and 4.890 ± 0.13 at 1 hr post injection respectively.
Shabnam Shahzad, Muhammad Abdul Qadir and Mahmood Ahmed
You can use below filter to find your desired issue