The rate of oxidation of thiamine ( Vitamin B, ) by Hg(II) ion in basic medium (pH=12.2) has been studied The concentration of [OH']and Hg(II) were kept constant by using 0.20M phosphate buffer and 2.0×10-3M Hg(II). The initial rate of formation of the only product of thiochrome was followed spectrophotometrically at h = 350 nm for the first 2-3 minutes The reaction has been studied in the temperature range of 279 K to 314.4 K. The reaction is pseudo-first order with respect to thiamine .Different kinetics and thermodynamics parameters such as rate costants, activation energy, enthalpy of activation, entropy of activation and Gibbs energy of activation of the reaction were reported. Various concentrations of thiamine were successfully found by measuring there initial rates in different synthetic mixtures containing other foreign species.
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