Induction of Selenium Nanoparticles Disturbs Behavior, Blood and Serum Biomarkers and Oxidative Stress Markers from Vital Organs of Male and Female Albino Mice

Current investigation was focused to determine the biological effects of Selenium nanoparticles (Se NPs) in mice. Se NPs (50mg/ml saline/Kg body weight) were intraperitoneally injected to 5 week old albino mice (N = 22) for 14 days. Control group was intraperitoneally injected with saline water (N = 22). In all subjects, a series of neurological tests, hematological parameters and markers of oxidative stress in vital organs were determined. We are reporting that rota rod and open field test performance remained unaffected in Se NPs injected mice when compared with saline treated controls. Male mice injected with Se NPs had significantly less line crossing (P = 0.02) while performing light dark box. They approached object A less frequently (P = 0.02) and spent lesser time with it (P = 0.001) during novel object recognition test (trial 1). % lymphocytes were significantly reduced (P = 0.03) in these mice while % monocytes were higher than control (P = 0.03). Concentration of cholesterol (P = 0.02) and LDL (P = 0.003) was significantly decreased in male mice. Female Se NPs treated mice spent less time (P = 0.05) with B object in trial 1 and 2 (P = 0.04) of novel object recognition test. They had significantly reduced cholesterol level (P = 0.02) and significantly increased catalase activity in the liver (P = 0.01) than control. Remaining parameters of behavior, blood chemistry and markers of oxidative stress from vital organs were non-significantly different upon their comparison between Se NPs and saline injected mice.

Mubashra Salim, Maria Shahzeen, Maryam Nasir Khan, Rimsha Tariq, Gul Muhammad, Ghulam Shabbir, Laraib Nisar, Muhammad Naeem Ashiq and Furhan Iqbal