A simple method was introduced for isothermal titration ca1orimctric data analysis. This method was applied to the binding of monosaccharide methyl α-D-mannoopyranoside to concava1in A at pH 6.9 and temperature of 25°C. The dissociation binding constant (K) and the molar enthalpy of binding (AU) were 0.135 mM and -28.47 kJ/mol, respectively. These results were markedly consistent with the results obtained from previous method.