Different sample treatment procedures such as dry ashing and wet digestion are commonly used for decompose biological materials and to bring the elements of interest into the measurable form. A number of reagents such aqua regia, HNO3/H2O2.HNO3/HCIO4 etc.are used to solubilize metals from biological matrix. In this study, performance of dry ashing and wet digestion methods has been compared for the estimination of zinc and calcium levels in the whole body freshwater fish samples. Flame atomic absorption spectrometry has been used as an analytical tool to quantity the metals. Result based on dry ashing and wet digestion methods for zinc differ significantly. Wet digestion methods give mostly higher zinc values compared to dry ashing methods which indicates the possible losses of zinc during the ashing of fish samples at elevated temperature, i.e. 500oC. higher recoveries of calcium in the whole body fresh water fish samples have been obtainted following wet digestion procedure using strontium as releasing agent compared to calcium determination following dry ashing method (with and without strontium) and wet digestion methods without strontium which suggests the use of a releasing agent (strontium) minimizes the chemical interferences of phosphate while determination calcium by flame atomic absorption spectrometry.
T M ANSARI ,S MAHBOOB ,T HANIF ,A SALAM ,M ARIF ,
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