Distribution of anthocyanins has been investigated in strawticrry fruit (Frugarks ananassr) at partially ripe and ripe levels. A fraction rich in anthocyanins was obtained by liquid-’ liquid extraction using acidic methanol (1: 99) followed by removal of lipids by n-hcxane. Lipid free extract wcs subjected to column chromatography to separate sugars and acids; whereas carotenoids were isolated from anthocyanins using TLC. The anthocyanin content represents 50.0 mg/ kg and 300 mg / kg in partially ripe and ripe fruit respectively; which is in agreement with thc literature data HPLC analysis was used to characterize individual anthocyanin and their relative concentration. Information from HPLC retention data revealed that major anthocyanin in partially ripe strawberry was pelargonidin 3eglucoside (33 mg / kg; II); whereas, cyanidin 3-’glucoside (152.6 mgI kg, 0 pelargonidin 3eglucoside (53.25 mg/ kg; II) and pelargonidin 3erutinoside (26.4 mg/ kg; III), were predominant anthocyanins in ripe strawberry fruit.
M ZAMIR ,M ASIF ,W AFZAL ,H SALEEM ,T FAZEELAT ,
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