In the current research work, (E)-N'-[1-(2,4-dihydroxyphenyl)ethylidene]substituted hydrazides were synthesized in a couple of steps and their enzyme inhibition potential was analyzed. Firstly 2,4-hydroxyacetophenone (1) was reacted with hydrated hydrazine (2) under stirring to yield (E)-4-(1-hydrazonoethyl)benzene-1,3-diol (3) which was further reacted with different acid halides, (4a-i) to afford (E)-[1-(2,4-dihydroxyphenyl)ethylidene]substituted hydrazides (5a-i). These synthesized compounds were characterized by EI-MS, 1H-NMR spectral techniques and were also evaluated against α-glucosidase and butyrylcholinesterase enzymes. The synthesized compounds were found to be acceptable inhibitors of α-glucosidase and decent inhibition against butyrylcholinesterase.

Muhammad Athar Abbasi, Syed Akhtar Hussain Shah, Aziz-ur-Rehman, Sabahat Zahra Siddiqui, Ghulam Hussain, Khalid Mohammed Khan, Muhammad Ashraf and Syeda Abida Ejaz