Statistical Analysis and Quantification of Alpha Tocopherol in Edible Seeds and Nuts of Pakistan by Reversed Phase HPLC with UV/Visible Detector

In present study, alpha tocopherol was determined in different edible seeds and nuts of Pakistani origin by reversed phase high performance liquid chromatography employing UV/Visible detector. Alpha tocopherol is an important constituent of vitamin E. Extraction of sample oil was carried out by Soxhlet method using n-hexane as a solvent. The maximum concentration of alpha tocopherol was found in wheat germs, i.e. 212.5 mg/100 g and minimum in cashews i.e. 0.15 mg/100 g. Almond, peanut, rice bran, corn, canola seeds, walnut, pine nuts and pistachios contained 37.8, 8.8, 3.6, 1.9, 1.6, 0.6, 0.26 and 0.2 mg of alpha tocopherol/100 g of sample respectively. The results show that the adequate amounts of alpha tocopherol are present in edible seeds and nuts, which emphasizes their recommendation in daily routine diet to remove the deficiency of vitamin E in human body.

WAHEED -UZ- ZAMAN ,MEHWISH AKRAM ,JAMIL ANWAR ,RABIA REHMAN ,