A flaw injection spectrophotometric method is described for the determination of choline based on the production of hydrogen peroxide from choline using immobilized choline oxidase mini-column. The product is mixed downstream to oxidize iodide into tiiodide at an elevated pH in the presence of molybdenum (VI), and the absorbance of the triiodide was monitored at 352 nm The detection limit is lower than 0.01 mmol/L with relative standard deviation of 1.5% over the range 0.1-1.0 mmol/L. The sample throughput was 40/h.


M YAQOOB ,A NABI ,M MASOOM YANSINZAI ,S H SHAH ,