Designing, Synthesis and Characterization of Fluorescent Fluorescein Hydrazides and Antibacterial Studies against Sinusitis Isolates and Hemolytic Activities

Fluorescein and its various derivatives show remarkable biological and bio-analytical applications because of its solubility, high degree of inertness and high fluorescent activity. To broaden the spectrum of its applications, we synthesized novel fluorescein-based hydrazides (FHJ-0.2 - FHJ-0.6) using different substituted aryl hydrazine. The newly designed and synthesized products have been monitored by thin layer chromatography and characterized by 1HNMR, IR and MS data. To find the application of these hydrazides, the biological screening is considered and screened by antimicrobial and hemolytic activity studies. Time-kill assay revealed that none of these hydrazides are appreciable antimicrobial agent but all of these show promising hemolytic results. Results are compared with Triton X-100 (positive control) with 89.54% hemolytic activity. Compared to fluorescein (FHJ-0.1) activity (3.07%), FHJ-0.3(3.54%), FHJ-0.2 (4.37%), FHJ-0.2 (4.37%) and FHJ-0.6 (5.87%), presented better anti- hemolytic activity showing that changing the substituent and position affects the activity remarkably. These fluoresecin based hydrazides may prove to be useful for the treatment of hemolytic disorders

Shazia Kousar, Abdul Jabbar, Muhammad Ahmad Mudassir, Syed Adnan Ali Shah, Fozia Anjum, Madiha Irfan, Ayesha Monawer and Syed Waqas Bukhari