Mutants of different auxotrophic nature of corynebacterium glutamicum were developed. Each mutant was further investigated for the production of lysine in three different fermentation media on Shake flask. The potency of each mutant for the conversion of consumed sugar to lysine and yield based on per gram dry cell weight was also examined. Mutant MRLH.GHAIO produced 28.64 gfllysine with 33.3% conversion and 3.36g lysine per gram dry cell weight in FMI medium and 26.35 glL lysine with 30.42% conversion and 2.77g lysine per gram dry cell weight in FM2 medium. The other auxotrophic mutant Lt. TJ. MT •. MTA2. H16, HA5 and GHJ produced 188-27.53 glL lysine with 24-31.7% conversion, 15.524.17 glL lysine with 17.8 -28.3% conversion in FM, and FM2 medium, respectively,All mutants showed least yield and least conversion efficiency in fermentation medium3 and higher yield and conversion efficiency then the parent thialysine resistant mutant TRI.
A H SHAH ,A HAMEED ,
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