A method of determination of five flavonoids in Saussurea involucrata by β-cyclodextrin modified capillary zone electrophoresis has been developed.The effects of buffer pH and buffer concentration, applied voltage and β-CD concentrations on the separation were systematically investigated. The optimum condition providing baseline separation of all compounds within 8 min was obtained in the 20 mmoll-1 borax buffer (pH 9.2), 20 kV applied voltage and 8 mmoll-1 β-CD. The linearity, detection limits, limits of quantification, reproducibility and recovery were satisfactory. The β-cyclodextrin modified capillary zone electrophoresis method proposed here has been satisfactorily employed to analyze S. involucrate samples.
Yumei Li and Hao Zhong
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