Trachyspermum ammi L, local seed’s essential oil and its crude methanolic extract were evaluated for antioxidant, antibacterial, and urease inhibition activities. Largely, 18 constituents were identified in the essential oil using The GC-MS. The promising constituent reported was terpeniodes; which seem to be responsible for the reported biological activities. DPPH Free radica1 scavenging assay was done to evaluate the antioxidant activities of both oil and crude extract. The DPPH scavenging activity results in lC50 values of 75 ± 0.05 and 300 ± 0.03. Antibacterial activity of the oil was carried out by using strains of Escherichia coli (89%), Bacillus subtilis (85%), and Streptococcus aureus (80%), while for the crude extract strains of E. coli (86%) B. subtle (89%), and S. aureus; (85%) were used. E. coil of T. ammi exhibits promising results with Aspergillus. flavus1 (86%) and Aspergillus1 Niger1 (87%). The phytochemical analysis of the T. ammi, crude extract showed the presence of saponins, flavonoids, steroids, terpenoids, and cardiac glycosides in the phytochemical investigation. Urease inhibition showed potent inhibition against B.P and J.B Urease with IC50 values of 85 ± 0.06 and 67.45 ± 0.8, respectively. Plant crude showed optimal results for B.P and Jack bean urease with IC50 values of 55.77 ± 0.01 and 35.56 ± 0.04, respectively.

Shahnaz, Hidayat Ullah Khan, Rani Begum, Fozia, Shaista Parveen and Afzal Shah