Enzymatic and spectrophotometric procedures are described for the quantitation of phosphorylcholine and phosphatidycholine based on the release of phosphate by acid phosphatase and phospholipase-C respectively. The phosphate produced enzymatically is complexed with molybdate in the presence of hydroquinone. Which is monitored at 720 run. The detection limit for both species was less than 0.01 mM with relative standard deviation of 0.4 - 1.2% in the range investigated. The method is applied to the determination of phosphatidylcholine in rat brain and phospholipase-D activity isolated from Pistachio khinjuk.
A NABI ,M MASOOM YASINZAI ,A SAEED ,M YAQOOB ,A WASEEM ,A K K ACHAKZAI ,
You can use below filter to find your desired issue