The seeds of Myristica fragrans (Myristicaceae) were studied for spermine. The fruit kernels (nutmegs) were subjected to chloroform extraction. Spermine was isolated by TLC using different solvent systems and flow injection micro column. Spermine acid adduct was prepared for quantitative determination of spermine. Absorbance was noted in visible region on spectrophotometer. Spermine was found to be 0.0051 % in fruit kernels of M. fragrans.
A B JASRA ,S A RANA ,A MAHMOOD ,M N KHAN ,
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