Different varieties of wheat grains from different ecological regions of Pakistan were evaluated for their molecular and physiochemical characteristics. Thirteen varieties examined included Tatara, Watan, Gandam-7l 1, Bakhtawar-92, Fakhre-Sarhad, Baldcar-0l, Ghaznawy, Saleem-2000, Zakht, Gandam-2002, Chudry-97, lnqilab-9l and Wafaq-01. The samples were evaluated for their variation in seed storage protein (gluten) by sodium dodecyl sulphate polycrylamide gel electrophoresis (SDS·PAGE) and for physiochemical characteristics including housand-grain mass (T GM), moisture contents and minerals contents. On the basis of molecular haracteristics, the variety Fakhre-Sarhad was designated as the best variety having higher number of molecular weight-Gluten subunits (HMW-GS), though still requiring improvement in the physiochemical status. On the contrary, Watan and gandam-7ll, saleem-2000 and Zakht and chudry-97 contained fewer number of HMW-GS as compared to Fakhre-Sarhad variety and were placed in second position. However, these varieties still need improvement in their protein level. The study may prove to be of great future importance in nutritional as well as crop improvement in Pakistan.