Lidocaine, a versatile analgesic, serves as a local anesthetic for topical dermal applications and as an agent to mitigate discomfort associated with chemotherapy in cancer patients. It is also utilized for postoperative pain management. Given its diverse applications, it is essential to investigate analytical and detection methods for lidocaine and its associated compounds, such as methylparaben. The present study introduces a simple and validated procedure for performing such analyses. The chromatographic system employed in this study comprised a GL Science Inc. Intersil ODS-3 column (150 mm length, 4.6 mm internal diameter, 5 µm particle size), with a mobile phase consisting of methanol:buffer (1:1), a detection wavelength of 220 nm, and operation at room temperature. A comprehensive validation study was conducted to confirm the accuracy, reproducibility, and precision of the results through the application of system suitability criteria. The UV-HPLC analytical method developed and validated in this research was evaluated for its capacity to detect low levels of lidocaine (Lido) and methylparaben (MP) within an efficient 8-minute run time. The limits of detection (LOD) were statistically determined as 6.636 µg/mL for Lido and 0.713 µg/mL for MP. The method demonstrated high recovery rates for both compounds, with an accuracy range of 98.7% to 101.1%. Furthermore, excellent linearity was observed, with coefficients of determination (R²) of 0.99992 for Lido and 0.99991 for MP.
Bandar R. Alsehli, Basima A.A. Saleem, Mahmoud Abdulnasser, and Mostafa F. Al-Hakkani
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