A new and sensitive spectrophotometric method for determination of micro amounts of barbituric acid with sodium nitrite is described. The method is applicable in acidic medium (pH 4.7) in the concentration range of 4-1600 mug/ml of barbituric acid. The reported method can be used for determination of barbituric acid in presence of various barbiturates which do not interfere.


RASHID IQBAL ,SHAHID M YOUSAF ,