Momordicacharantia L. belongs to the family Cucurbitaceae. This plant has widely been reported for use in traditional medical systems for the treatment of diabetes, rheumatism, intestinal gas and malaria. The ethnicity of M. charantiais Asia, Africa and some parts of Europe where it is considered edible. Sewage sludge, a by-product of waste water treatment plant, contains several nutrients. These nutrients are known as beneficial for the better growth and good yield of the plants. The objective of this study is to optimize soil with different ratios of sewage sludge for increased growth of bioactive components in M. charantia. The plant was grown at six places; in soil containing 20%, 40%, 60% and 80% sewage sludge ratio as well as in pure soil and in pure sewage sludge. Fruit harvesting was carried out after 75 days. Methanol extract of M. charantia (Fruit) was subjected to analysis using GC-MS. Comparative quantification of 7,11-hexadecadienal, oleic Acid and stigmasterol were carried out. It has been found that the soil containing 60% sewage sludge yielded maximum quantity of 7,11-hexadecadienal, oleic Acid and stigmasterol. Therefore, optimization of soil with different ratios of sewage sludge is carried out and 60% ratio of sewage sludge in soil is recommended for the promising growth of Momordicacharantia.

Zohaib Saeed, Ikram Ahmad, Shahid Iqbal, Umer Younas, Muhammad Pervaiz, Rana Rashad Mahmood Khan, Shahzad Sharif, Abdullah M. Asiri, Sher Bahadar Khan and Tahseen Kamal