A flow injection analysis method for blood urea has been described in combination with a modified poly (vinyl chloride)-nonaction potentiometric ammonium ion transducer. The urease enzyme has been effectively immobilized on a 5 mm trinitrate cellulose membrane by covalent cross linking. Reusability, stability and durability regarding the activity of the immobilized enzyme has been successfully demonstrated. It has been proposed that such membrane filters can be an effective base material in comparison to a column type enzyme reactor for immobilization of enzyme due to their large available hollow space.
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