Separation of Copper I and II Iron II and III in Composite Aqueous Matrix by Compititive Chelation Using Disc Electrophoresis Technique

A  new, simple   and rapid  Disc electrophoresis  method  has been developed   in  our laboratory for  determination of different oxidation states of Iron and  copper singly as well   as  in mixture  form  through  the specially   prepared  glass  tubes   of polacrylamide   gel   using  glycine   buffer  at  pH  4  and  3.5. For quantitative analysis   the intensity of electrophoratically separated colored   bands   of   respective   metal   ions in   the   tubes   were    measured   using   Thin   layer chromatographic scanner CS-910.  Ne developed plates      were scanned   separately in UV,   Vis and   fluorescence    regions   on    pre-determined       wavelengths   simultaneously   integration   and profile mode.   It has  been  observed that,  very sharp  Disc  bands  with particular  colour  of both cations with  different  oxidation  state were  obtained   with approximately ten  micron  thickness. The detection limits has been achieved in the range of mg/µl level.

M A JAKHRANI ,T G KAZI ,M F KHAH ,M H S WATTOO ,G H KAZI ,